Monitoring Indicators of the NETPHARM Project
Legend: Achieved by June 30, 2025 / Target
Modernised Laboratories – Instruments
Charles University, Faculty of Pharmacy in Hradec Králové
Circular dichroism spectrometer with single position Peltier thermostatted cell holder, X-ray diffractometer, Isolator DNA, Krumdieck Tissue Slicer, Confocal Microscopy Workstation, Telemetric measurement of arterial blood pressure, UV/Vis/NIR spektrometr, Capillary electrophoresis for nucleic acid separation and nanopore sequencer, Data Storage
Charles University, Faculty of Medicine in Hradec Králové
Ultracentrifuge
University of Chemistry and Technology, Prague
Ultra-performance liquid chromatography
Institute of Macromolecular Chemistry of the CAS
Helium recovery system, Thermostat with stainless steel bath and cooling, High-speed digital camera with connection to optical microscopes
CEITEC Masaryk University
Computing station, GPU/CPU server
Key Publications
The farnesoid X receptor (FXR) antagonist 7β-isopropylchenodeoxycholic acid improves glucose metabolism in mice on a Western diet
Alzbeta Stefela, Klara Dohnalova, Hana Lastuvkova, Josef Skoda, Maria Bajnokova, Sophie Lestavel, Veronique Touche, Miroslav Kaspar, Eva Kudova, Milos Hroch, Stanislav Micuda, Otto Kucera, Petr Pavek
DOI: https://doi.org/10.1016/j.phrs.2025.107950
The hypolipidemic effect of MI-883, the combined CAR agonist/ PXR antagonist, in diet-induced hypercholesterolemia model
Jan Dusek, Ivana Mejdrová, Klára Dohnalová, Tomas Smutny, Karel Chalupsky, Maria Krutakova, Josef Skoda, Azam Rashidian, Ivona Pavkova, Kryštof Škach, Jana Hricová, Michaela Chocholouskova, Lucie Smutna, Rajamanikkam Kamaraj, Miloš Hroch, Martin Leníček, Stanislav Mičuda, Dirk Pijnenburg, Rinie van Beuningen, Michal Holčapek, Libor Vítek, Magnus Ingelman-Sundberg, Oliver Burk, Thales Kronenberger, Radim Nencka & Petr Pavek
DOI: https://doi.org/10.1038/s41467-025-56642-y
Topobexin targets the Topoisomerase II ATPase domain for beta isoform-selective inhibition and anthracycline cardioprotection
Jan Kubeš, Galina Karabanovich, Anh T. Q. Cong, Iuliia Melnikova, Olga Lenčová, Petra Kollárová, Hana Bavlovič Piskáčková, Veronika Keresteš, Lenka Applová, Lise C. M. Arrouye, Julia R. Alvey, Jasmina Paluncic, Taylor L. Witter, Anna Jirkovská, Jiří Kuneš, Petra Štěrbová-Kovaříková, Caroline A. Austin, Martin Štěrba, Tomáš Šimůnek, Jaroslav Roh & Matthew J. Schellenberg
DOI: https://doi.org/10.1038/s41467-025-60167-9
Sulfonated Hydroxyaryl-Tetrazines with Increased pKa for Accelerated Bioorthogonal Click-to-Release Reactions in Cells
Michal Rahm, Patrick Keppel, Dr. Veronika Šlachtová, Dr. Rastislav Dzijak, Dr. Martin Dračínský, Simona Bellová, Dr. Paul E. Reyes-Gutiérrez, Dr. Sille Štěpánová, Jakob Raffler, Eva Tloušťová, Dr. Helena Mertlíková-Kaiserová, Dr. Hannes Mikula, Dr. Milan Vrabel
DOI: https://doi.org/10.1002/anie.202411713
Transforming Aryl-Tetrazines into Bioorthogonal Scissors for Systematic Cleavage of trans-Cyclooctenes
Dr. Martin Wilkovitsch, Dr. Walter Kuba, Patrick Keppel, Dr. Barbara Sohr, Andreas Löffler, Dr. Stefan Kronister, Andres Fernandez del Castillo, Dr. Marion Goldeck, Dr. Rastislav Dzijak, Michal Rahm, Dr. Milan Vrabel, Dr. Dennis Svatunek, Prof. Dr. Jonathan C. T. Carlson, Prof. Dr. Hannes Mikula
DOI: https://doi.org/10.1002/anie.202411707
Improved electro-destruction of bacterial biofilms by coating conductive surfaces with polymers
Stephane Hoang, Hanna Zhukouskaya, Iryna Ivanko, Jan Svoboda, Michaela Hympanov, Jan Marek, Ondřej Soukup, Miroslav Slouf, Jan Kotek, Eric Doris, Edmond Gravel, Elena Tomšík, Martin Hrubý
DOI: https://doi.org/10.1016/j.eurpolymj.2024.112910
pH-induced morphological reversible transition from microparticles to vesicles for effective bacteria entrapment
Vladimir Sincari, Svetlana Lukáš Petrova, Eliézer Jäger, Alessandro Jäger, Rafał Konefał, Veronika Gajdošová, Miroslav Šlouf, Hympánová Michaela, Jan Marek, Ondrej Soukup, Martin Hrubý
DOI: https://doi.org/10.1016/j.eurpolymj.2024.113511
The role of coatings and plasticizers compatibility in stability of advanced gastro-resistant self-emulsifying pellets designed for the delivery of volatile compounds: A combined solid-state NMR and dissolution study
Jan Macku, Jakub Vyslouzil, Jan Muselik, Miroslava Pavelkova, David Vetchy, Daniela Hlavata, Miroslav Slouf, Martina Urbanova, Jiri Brus, Katerina Kubova
DOI: https://doi.org/10.1016/j.jddst.2024.106325
BAPTA-based potentiometric polymer sensor: towards sensing inflammations and infections
Nikol Janić, Hanna Zhukouskaya, Peter Černoch, Jiří Pánek, Jan Svoboda, Milena Hajná, Alena Řezníčková, Elena Tomšík, Martin Hrubý
DOI: https://doi.org/10.1039/D4TB02586E
Prevalence, country-specific prescribing patterns and determinants of benzodiazepine use in community-residing older adults in 7 European countries
Lukačišinová A, Reissigová J, Ortner-Hadžiabdić M, Brkic J, Okuyan B, Volmer D, Tadić I, Modamio P, Mariño EL, Tachkov K, Liperotti R, Onder G, Finne-Soveri H, van Hout H, Howard EP, Fialová D.
DOI: https://doi.org/10.1186/s12877-024-04742-7
Polypharmacy and potentially inappropriate prescribing of benzodiazepines in older nursing home residents.
Kummer I, Reissigová J, Lukačišinová A, Ortner Hadžiabdić M, Stuhec M, Liperoti R, Finne-Soveri H, Onder G, van Hout H, Fialová D.
DOI: https://doi.org/10.1080/07853890.2024.2357232
The effects of diuretic deprescribing in adult patients: A systematic review to inform an evidence-based diuretic deprescribing guideline
van Poelgeest E, Paoletti L, Özkök S, Pinar E, Bahat G, Vuong V, Topinková E, Daams J, McCarthy L, Thompson W, van der Velde N.
DOI: https://doi.org/10.1111/bcp.16189
Interdisciplinární spolupráce klinických farmaceutů, praktických lékařů a geriatrů v individualizované farmakoterapii ve stáří – novinky v oblasti vzdělávání, výzkumu a klinické praxe
Fialová Daniela Topinková Eva, Jurašková Božena, Matějková Astrid, Antonenko Olena, Sulková-Dusilová Sylvie.
Practicus. 2024; 7: 17-25
Chronický renokardiální syndrom u seniorů – pohled nefrologa a poznámky pro praxi. Gero a Geri
Sulková S, Jurašková B, Fialová D, Kladlec M., Šafránek R., Topinková E.
Gero a Geri 2024; 133 (3): 134-141
Rifampicin and its derivatives: stability, disposition, and affinity towards pregnane X receptor employing 2D and 3D primary human hepatocytes
Tomas Smutny, Lucie Smutna, Lukas Lochman, Rajamanikkam Kamaraj, Radim Kucera, Petr Pavek
DOI: https://doi.org/10.1016/j.bcp.2024.116500
Quantifying expression and metabolic activity of genes regulated by pregnane X receptor in primary human hepatocyte spheroids
Lukáš Lochman, Ellen Tanaka Kahiya, Bechara Saade, Tomáš Smutný, Jurjen Duintjer Tebbens, Petr Pávek, Veronika Bernhauerová
DOI: https://doi.org/10.1371/journal.pcbi.1012886
Environment-like exposure of free-living stages of Haemonchus contortus to albendazole: The intra- and intergeneration stability of altered expression of UDP-glycosyl transferases and ABC-transporters
Karolína Štěrbová, Linh Nguyen Thuy, Martin Žofka, Petra Matoušková, Josef Krátký, Lenka Skálová
DOI: https://doi.org/10.1016/j.chemosphere.2025.144281
Significant Follow-up National Grants
Project Title: How are drug-metabolising enzymes regulated in parasitic nematodes?
Granting Authority: Czech Science Foundation
Duration: 1 January 2025 – 31 December 2027
Principal Investigatore: Assoc. Prof. Ing. Petra Matoušková, Ph.D., Charles University / Faculty of Pharmacy in Hradec Králové
Co-Investigator: RNDr. Martin Kašný, Ph.D., Masaryk University / Faculty of Science
https://www.isvavai.cz/cep?s=jednoduche-vyhledavani&ss=detail&n=0&h=GA25-16530S
Project Title: Development of a comprehensive prediction model and software tool for supercritical fluid chromatography using artificial neural networks
Granting Authority: Czech Science Foundation
Duration: 1 January 2025 – 31 December 2027
Principal Investigator: Prof. PharmDr. Lucie Nováková, Ph.D., Charles University / Faculty of Pharmacy in Hradec Králové
https://www.isvavai.cz/cep?s=jednoduche-vyhledavani&ss=detail&n=0&h=GA25-15354S
Project Title: Understanding the mechanisms of formation, damage, and restoration of the epidermal lipid barrier: implications for skin diseases
Granting Authority: Czech Science Foundation
Duration: 1 January 2025 – 31 December 2027
Principal Investigator: Assoc. Prof. Mgr. Jarmila Zbytovská, Dr. rer. nat., University of Chemistry and Technology, Prague / Faculty of Chemical Technology
Co-Investigator: Prof. PharmDr. Kateřina Vávrová, Ph.D., Charles University / Faculty of Pharmacy in Hradec Králové
https://www.isvavai.cz/cep?s=jednoduche-vyhledavani&ss=detail&n=0&h=GA25-17203S
Project Title: An overlooked phenomenon with a significant impact – chirality of (metalla)carborane clusters
Granting Authority: Czech Science Foundation
Duration: 1 January 2025 – 31 December 2027
Principal Investigator: RNDr. Bohumír Grüner, CSc., Institute of Inorganic Chemistry of the Czech Academy of Sciences
Co-Investigator: Assoc. Prof. PharmDr. Radim Kučera, Ph.D., Charles University / Faculty of Pharmacy in Hradec Králové
https://www.isvavai.cz/cep?s=jednoduche-vyhledavani&ss=detail&n=0&h=GA25-16216S
Significant Follow-up International Grants
Project Title: New Approaches to the Prevention and Therapy of Tick-Borne Encephalitis and Other Flavivirus Infections
Granting Authority: Ministry of Education, Youth and Sports, INTER-EXCELLENCE II programme
Duration: 1 March 2025 – 31 December 2028
Principal Investigator: Assoc. Prof. RNDr. Luděk Eyer, Ph.D., Veterinary Research Institute
Co-Investigator: RNDr. Milan Dejmek, Ph.D., Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences
https://www.isvavai.cz/cep?s=jednoduche-yhledavani&ss=detail&n=0&h=LUAUS25011
Patent Applications Filed
DNA TOPOISOMERASE IIB INHIBITORS (zn. PCT/US2024/043047), Authors: Jaroslav Roh (UK); Tomáš Šimůnek (UK); Martin Štěrba (UK)
„The unique inhibitory and cardioprotective properties of the topoisomerase II inhibitors, which were developed within the VZ1 research programme (Roh), were discovered during research in the VZ2 programme (Šimůnek, Štěrba). For this reason, an international PCT patent application was filed.“
